Gated is a free, light-weight and elegant analyser and visualizer of FCS data. It is a cross-platform application with supports of:
- Management of FCS samples into groupings and workspace.
- Spillover compensation
- Hierarchial gating
- Common statistical analysis of sub-populations
- Plotting and visual style customization
- Data normalization, integration and dimensionality reduction built-in
- Layout editor for graphics export
- Automation, macro, and scripting
- Free access to Python backend through scripting engine
- Embedded data package format for reproducible analysis and sharing
See the releases for compiled binary packages for Windows x64, macOS ARM64, macOS x64 and Linux x64 (.NET 10) Download Releases. .NET runtime installation is required to install manually rather than packed.
This is the portable software package that you could decompress anywhere without administrator previlege and run the application. The software will try to update itself if newer versions are released if there are network connection from the GitHub repository releases.
This application bundles with a copy of embedded Python runtime. For Windows, its machinary of library finding and isolation makes it works fine, however, for UNIX like operating system, we disabled PATH finding to independently isolate the Python environment. However, this will make it fail to locate system compilers when compiling package sources. (Also, for UMAP and Numba for example, CMake is required). These dependencies should be resolved by user and installed and configured manually.
Population hierarchy
Layout editor
Data normalization and integration
Scripting
Gated is licensed under GNU GPLv3. This program is distributed in the hope that it will be useful, but without any warranty; without even the implied warranty of merchantability or fitness for a particular purpose. See the GNU General Public License for more details.
Copyright (C) Zheng Yang 2025 - 2026.